Enzymatic Synthesis of Highly Fluorescent 8-Azapurine Ribosides Using a Purine Nucleoside Phosphorylase Reverse Reaction: Variable Ribosylation Sites

Various forms of purine-nucleoside phosphorylase (PNP) were used as catalysts of enzymatic ribosylation of selected fluorescent 8-azapurines. It was found that the recombinant calf PNP catalyzes ribosylation of 2,6-diamino-8-azapurine in a phosphate-free medium, with ribose-1-phosphate as ribose don...

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Библиографические подробности

Главные авторы:	Goran Mikleušević, Alicja Stachelska-Wierzchowska, Beata Wielgus-Kutrowska, Jacek Wierzchowski
Формат:	Artigo
Язык:	Inglês
Опубликовано:	MDPI AG 2013-10-01
Серии:	Molecules
Предметы:	8-azapurines nucleosides enzymatic synthesis fluorescence purine-nucleoside phosphorylase
Online-ссылка:	http://www.mdpi.com/1420-3049/18/10/12587
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