Protocol for live-cell fluorescence-guided cryoFIB-milling and electron cryo-tomography of virus-infected cells
Summary: Here, we present a protocol for assessing virus-infected cells using electron cryo-tomography (cryoET). It includes the basic workflows of seeding cells, plunge-freezing, clipping, cryo-focused ion beam milling (cryoFIB-milling), and cryoET, as well as two optional modules: micropatterning...
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| Principais autores: | , , , |
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| 格式: | Artigo |
| 语言: | Inglês |
| 出版: |
Elsevier
2022-12-01
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| 丛编: | STAR Protocols |
| 主题: | |
| 在线阅读: | http://www.sciencedirect.com/science/article/pii/S2666166722005767 |
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