Super-resolution confocal cryo-CLEM with cryo-FIB milling for in situ imaging of Deinococcus radiodurans

Studying bacterial cell envelope architecture with electron microscopy is challenging due to the poor preservation of microbial ultrastructure with traditional methods. Here, we established and validated a super-resolution cryo-correlative light and electron microscopy (cryo-CLEM) method, and combin...

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Detalhes bibliográficos

Principais autores:	Danielle L. Sexton, Steffen Burgold, Andreas Schertel, Elitza I. Tocheva
Formato:	Artigo
Idioma:	Inglês
Publicado em:	Elsevier 2022-01-01
coleção:	Current Research in Structural Biology
Assuntos:	Cryo-CLEM Cryo-FIB Cryo-ET Cryo-super resolution microscopy Microbial ultrastructure Cell envelope architecture
Acesso em linha:	http://www.sciencedirect.com/science/article/pii/S2665928X21000295
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