In Vivo Protein–Protein Binding Competition Assay Based on Split-GFP Reassembly: Proof of Concept

The split-green fluorescent protein (GFP) reassembly assay is a well-established approach to study protein–protein interactions (PPIs). In this assay, when two interacting proteins X and Y, respectively fused to residues 1–157 and to residues 158–237 of GFP, are co-expressed in <i>E. coli</i>, the t...

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Үндсэн зохиолчид:	Christophe Bignon, Sonia Longhi
Формат:	Artigo
Хэл сонгох:	Inglês
Хэвлэсэн:	MDPI AG 2023-02-01
Цуврал:	Biomolecules
Нөхцлүүд:	protein complementation assays protein–protein interactions intrinsically disordered proteins fluorescence binding competition assays
Онлайн хандалт:	https://www.mdpi.com/2218-273X/13/2/354
Шошгууд:	Шошго нэмэх Шошго байхгүй, Энэхүү баримтыг шошголох эхний хүн болох!