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Protocol for adhesion and immunostaining of lymphocytes and other non-adherent cells in culture

Immunostaining of non-adherent cells is commonly performed after adhesion of cells onto microscope slides either using cytocentrifugation or with the help of charged coating substrates. These techniques, however, require either specialized equipment or significant preparation time. Here, we describe...

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Hlavní autoři: Matthew Tsang, Jennifer Gantchev, Feras M. Ghazawi, Ivan V. Litvinov
Médium: Artigo
Jazyk:Inglês
Vydáno: Taylor & Francis Group 2017-11-01
Edice:BioTechniques
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On-line přístup:https://www.future-science.com/doi/10.2144/000114610
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