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Protocol for adhesion and immunostaining of lymphocytes and other non-adherent cells in culture

Immunostaining of non-adherent cells is commonly performed after adhesion of cells onto microscope slides either using cytocentrifugation or with the help of charged coating substrates. These techniques, however, require either specialized equipment or significant preparation time. Here, we describe...

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Autors principals: Matthew Tsang, Jennifer Gantchev, Feras M. Ghazawi, Ivan V. Litvinov
Format: Artigo
Idioma:Inglês
Publicat: Taylor & Francis Group 2017-11-01
Col·lecció:BioTechniques
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Accés en línia:https://www.future-science.com/doi/10.2144/000114610
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