Superloser: A Plasmid Shuffling Vector for Saccharomyces cerevisiae with Exceedingly Low Background

Here we report a new plasmid shuffle vector for forcing budding yeast (Saccharomyces cerevisiae) to incorporate a new genetic pathway in place of a native pathway – even an essential one – while maintaining low false positive rates (less than 1 in 108 per cell). This plasmid, dubbed “Superloser,” wa...

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Bibliografske podrobnosti

Principais autores:	Max A. B. Haase, David M. Truong, Jef D. Boeke
Format:	Artigo
Jezik:	Inglês
Izdano:	Oxford University Press 2019-08-01
Serija:	G3: Genes, Genomes, Genetics
Teme:	S. cerevisiae plasmid shuffling 5-FOA counterselection humanization
Online dostop:	http://g3journal.org/lookup/doi/10.1534/g3.119.400325
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