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Superloser: A Plasmid Shuffling Vector for Saccharomyces cerevisiae with Exceedingly Low Background

Here we report a new plasmid shuffle vector for forcing budding yeast (Saccharomyces cerevisiae) to incorporate a new genetic pathway in place of a native pathway – even an essential one – while maintaining low false positive rates (less than 1 in 108 per cell). This plasmid, dubbed “Superloser,” wa...

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Principais autores: Max A. B. Haase, David M. Truong, Jef D. Boeke
Format: Artigo
Sprog:Inglês
Udgivet: Oxford University Press 2019-08-01
Serier:G3: Genes, Genomes, Genetics
Fag:
S. cerevisiae
plasmid shuffling
5-FOA counterselection
humanization
Online adgang:http://g3journal.org/lookup/doi/10.1534/g3.119.400325
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