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Scaling the manufacture of adipose tissue-derived mesenchymal stromal cells: integrated bioreactor workflows from inoculation to harvest without the need of seed train

Abstract Background The production of human mesenchymal stromal cells (hMSC) for therapeutic use requires scalable, efficient and standardized manufacturing processes that could further benefit from shortening manufacturing time, automate and simplify operations with closed systems. This study aimed...

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Principais autores: Marta H. G. Costa, Ana Paula Terrasso, Inês E. Crespo, Raúl Valero, Beatriz Menéndez, Beatriz Painho, Carolina D. Sousa, Beatriz Gamelas, Hélio Tomás, Pablo Mancheno-Corvo, Ramón Menta, Carmen R. Gugel, Vanessa Fernández-Gómez, Ángel Herrero-Méndez, Irene Zamora Mármol, Alvaro Avivar-Valderas, Laura M. Pérez, Olga de la Rosa, Eleuterio Lombardo, Margarida Serra, Maitane Ortiz-Virumbrales
Formato: Artigo
Idioma:Inglês
Publicado em: BMC 2026-01-01
coleção:BMC Biotechnology
Assuntos:
Mesenchymal stromal cells
Bioreactors
No seed train
Microcarriers
Closed bioprocess
Xeno-free medium
Acesso em linha:https://doi.org/10.1186/s12896-026-01101-9
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