Development and application of a quadruple RT-qPCR assay for the simultaneous detection of NoV GI, NoV GII, and HAV in bivalve shellfish

ABSTRACT To achieve rapid and simultaneous detection of NoV GI, NoV GII, and HAV, a quadruple real-time fluorescence quantitative PCR (RT-qPCR) assay was developed using MS2 bacteriophage as a process control virus. The quadruple RT-qPCR assay effectively detected NoV GI, NoV GII, HAV, and MS2 RNA w...

Ausführliche Beschreibung

Bibliografische Detailangaben

Hauptverfasser:	Yan Wang, Jinfeng Wang, Maolin Wei, Libing Liu, Jianchang Wang, Xiangdong Xu
Format:	Artigo
Sprache:	Inglês
Veröffentlicht:	American Society for Microbiology 2025-01-01
Schriftenreihe:	Applied and Environmental Microbiology
Schlagworte:	noroviruses hepatitis A virus quadruple RT-qPCR simultaneous detection bivalve shellfish MS2
Online-Zugang:	https://journals.asm.org/doi/10.1128/aem.01839-24
Tags:	Tag hinzufügen Keine Tags, Fügen Sie das erste Tag hinzu!