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Development and application of a quadruple RT-qPCR assay for the simultaneous detection of NoV GI, NoV GII, and HAV in bivalve shellfish

ABSTRACT To achieve rapid and simultaneous detection of NoV GI, NoV GII, and HAV, a quadruple real-time fluorescence quantitative PCR (RT-qPCR) assay was developed using MS2 bacteriophage as a process control virus. The quadruple RT-qPCR assay effectively detected NoV GI, NoV GII, HAV, and MS2 RNA w...

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Principais autores: Yan Wang, Jinfeng Wang, Maolin Wei, Libing Liu, Jianchang Wang, Xiangdong Xu
Format: Artigo
Sprog:Inglês
Udgivet: American Society for Microbiology 2025-01-01
Serier:Applied and Environmental Microbiology
Fag:
noroviruses
hepatitis A virus
quadruple RT-qPCR
simultaneous detection
bivalve shellfish
MS2
Online adgang:https://journals.asm.org/doi/10.1128/aem.01839-24
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