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A specific, stable, and accessible LAMP assay targeting the HSP70 gene of Trypanosoma cruzi

ABSTRACT Diagnostic delays prevent most Chagas disease patients from receiving timely therapy during the acute phase when treatment is effective. Quantitative PCR (qPCR)-based diagnostic methods provide high sensitivity during this phase but require specialized equipment and complex protocols. More...

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Main Authors: Sneider Alexander Gutierrez Guarnizo, Beth Jessy Condori, Luciana Basma, Shirley Equilia, Edith Malaga, Siena Defazio, Emily Arteaga, Jean Karla Velarde, Martín Obregón, Anshule Takyar, Carolina Duque, Jill Hakim, Freddy Tinajeros, Robert H. Gilman, Natalie Bowman, Monica R. Mugnier
Format: Artigo
Language:Inglês
Published: American Society for Microbiology 2025-11-01
Series:Microbiology Spectrum
Subjects:
molecular diagnosis
LAMP-PCR
cost-effective method
HSP70 gene
acute chagas
congenital transmission
Online Access:https://journals.asm.org/doi/10.1128/spectrum.00172-25
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