Stepwise error‐prone PCR and DNA shuffling changed the pH activity range and product specificity of the cyclodextrin glucanotransferase from an alkaliphilicBacillus sp
Cyclodextrin glucanotransferase (EC 2.4.1.19) from the alkaliphilicBacillus sp. G‐825‐6 converts starch mainly to γ‐cyclodextrin (CD8). A combination of error‐prone PCR and DNA shuffling was used to obtain variants of this enzyme with higher product specificity for CD8 and a broad pH activity range....
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| Principais autores: | , , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado: |
Wiley
2015-01-01
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| Series: | FEBS Open Bio |
| Assuntos: | |
| Acceso en liña: | https://doi.org/10.1016/j.fob.2015.06.002 |
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