A simple enrichment procedure improves detection of membrane proteins by immunoblotting
We developed a novel approach to improve detection of membrane-associated proteins in yeast cell lysates by immunoblotting. Our method consists of a simple enrichment procedure using sedimentation to remove soluble proteins and the use of an alternative electrophoresis sample buffer, which allows fo...
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| Principais autores: | , |
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| Format: | Artigo |
| Sprog: | Inglês |
| Udgivet: |
Taylor & Francis Group
2016-11-01
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| Serier: | BioTechniques |
| Fag: | |
| Online adgang: | https://www.future-science.com/doi/10.2144/000114474 |
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