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A simple enrichment procedure improves detection of membrane proteins by immunoblotting

We developed a novel approach to improve detection of membrane-associated proteins in yeast cell lysates by immunoblotting. Our method consists of a simple enrichment procedure using sedimentation to remove soluble proteins and the use of an alternative electrophoresis sample buffer, which allows fo...

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Hlavní autoři: Azamat Karginov, Michael Agaphonov
Médium: Artigo
Jazyk:Inglês
Vydáno: Taylor & Francis Group 2016-11-01
Edice:BioTechniques
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On-line přístup:https://www.future-science.com/doi/10.2144/000114474
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