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Epitope Mapping of a Monoclonal Antibody Directed against Neisserial Heparin Binding Antigen Using Next Generation Sequencing of Antigen-Specific Libraries.

We explore here the potential of a newly described technology, which is named PROFILER and is based on next generation sequencing of gene-specific lambda phage-displayed libraries, to rapidly and accurately map monoclonal antibody (mAb) epitopes. For this purpose, we used a novel mAb (designated 31E...

Ausführliche Beschreibung

Gespeichert in:
Hauptverfasser: Maria Domina, Veronica Lanza Cariccio, Salvatore Benfatto, Mario Venza, Isabella Venza, Danilo Donnarumma, Erika Bartolini, Erica Borgogni, Marco Bruttini, Laura Santini, Angelina Midiri, Roberta Galbo, Letizia Romeo, Francesco Patanè, Carmelo Biondo, Nathalie Norais, Vega Masignani, Giuseppe Teti, Franco Felici, Concetta Beninati
Format: Artigo
Sprache:Inglês
Veröffentlicht: Public Library of Science (PLoS) 2016-01-01
Schriftenreihe:PLoS ONE
Online-Zugang:http://europepmc.org/articles/PMC4980009?pdf=render
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