Validation of picogram- and femtogram-input DNA libraries for microscale metagenomics

High-throughput sequencing libraries are typically limited by the requirement for nanograms to micrograms of input DNA. This bottleneck impedes the microscale analysis of ecosystems and the exploration of low biomass samples. Current methods for amplifying environmental DNA to bypass this bottleneck...

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Principais autores:	Christian Rinke, Serene Low, Ben J. Woodcroft, Jean-Baptiste Raina, Adam Skarshewski, Xuyen H. Le, Margaret K. Butler, Roman Stocker, Justin Seymour, Gene W. Tyson, Philip Hugenholtz
格式:	Artigo
語言:	Inglês
出版:	PeerJ Inc. 2016-09-01
叢編:	PeerJ
主題:	Nextera XT 100 fg Low input DNA library Picogram Reagent contamination Low biomass
在線閱讀:	https://peerj.com/articles/2486.pdf
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