Probing the SERCA1a sarcoplasmic reticulum Ca2+-ATPase phosphorylation-site mutant D351E with inorganic phosphate

The expression of sarcoplasmic reticulum SERCA1a Ca2+-ATPase wild-type and D351E mutants was optimized in yeast under the control of a galactose promoter. Fully active wild-type enzyme was recovered in yeast microsomal membrane fractions in sufficient amounts to permit a rapid and practical assay of...

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Autori principali:	A.C.O. Carreira, C.M.V. Bastos, S. Verjovski-Almeida
Natura:	Artigo
Lingua:	Inglês
Pubblicazione:	Associação Brasileira de Divulgação Científica 2007-10-01
Serie:	Brazilian Journal of Medical and Biological Research
Soggetti:	Phosphorylation site mutant Catalytic site Yeast expression system Saccharomyces cerevisiae P-type ATPase
Accesso online:	http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2007001000004
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