Probing the SERCA1a sarcoplasmic reticulum Ca2+-ATPase phosphorylation-site mutant D351E with inorganic phosphate

The expression of sarcoplasmic reticulum SERCA1a Ca2+-ATPase wild-type and D351E mutants was optimized in yeast under the control of a galactose promoter. Fully active wild-type enzyme was recovered in yeast microsomal membrane fractions in sufficient amounts to permit a rapid and practical assay of...

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Detaylı Bibliyografya
Asıl Yazarlar:	A.C.O. Carreira, C.M.V. Bastos, S. Verjovski-Almeida
Materyal Türü:	Artigo
Dil:	Inglês
Baskı/Yayın Bilgisi:	Associação Brasileira de Divulgação Científica 2007-10-01
Seri Bilgileri:	Brazilian Journal of Medical and Biological Research
Konular:	Phosphorylation site mutant Catalytic site Yeast expression system Saccharomyces cerevisiae P-type ATPase
Online Erişim:	http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2007001000004
Etiketler:	Etiketle Etiket eklenmemiş, İlk siz ekleyin!

Probing the SERCA1a sarcoplasmic reticulum Ca2+-ATPase phosphorylation-site mutant D351E with inorganic phosphate

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