Monitoring Nuclear Import with GFP-Variant Fusion Proteins in Digitonin-Permeabilized Cells
A convenient assay for monitoring nuclear localization signal-mediated nuclear import of green fluorescent protein (GFP)-variant fusion proteins has been developed. This modified assay relies upon indirect immunofluorescence microscopy for visualization of transported substrates. The use of GFP-vari...
Na minha lista:
| Principais autores: | , |
|---|---|
| Format: | Artigo |
| Sprog: | Inglês |
| Udgivet: |
Taylor & Francis Group
2001-10-01
|
| Serier: | BioTechniques |
| Online adgang: | https://www.future-science.com/doi/10.2144/01314st02 |
| Tags: |
Ingen Tags, Vær først til at tagge denne postø!
|