Monitoring Nuclear Import with GFP-Variant Fusion Proteins in Digitonin-Permeabilized Cells
A convenient assay for monitoring nuclear localization signal-mediated nuclear import of green fluorescent protein (GFP)-variant fusion proteins has been developed. This modified assay relies upon indirect immunofluorescence microscopy for visualization of transported substrates. The use of GFP-vari...
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| Autors principals: | , |
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| Format: | Artigo |
| Idioma: | Inglês |
| Publicat: |
Taylor & Francis Group
2001-10-01
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| Col·lecció: | BioTechniques |
| Accés en línia: | https://www.future-science.com/doi/10.2144/01314st02 |
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