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Purification and characterization of recombinant FAD synthetase from Neurospora crassa

FAD Synthetase (FADS) [EC 2.7.7.2], the second enzyme in flavin cofactor biosynthetic pathway converts FMN to FAD, plays an important role in many redox reactions. Neurospora crassa FADS (NcFADS) was cloned and overexpressed in E. coli cells. Recombinant NcFADS was purified in high yields of ∼8 mg p...

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Auteurs principaux: Nirupama Puvvada, Sridhar Gunde, Ch. Venkata Ramana Devi, Raghu Gogada
Format: Artigo
Langue:Inglês
Publié: Elsevier 2021-12-01
Collection:Biochemistry and Biophysics Reports
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Accès en ligne:http://www.sciencedirect.com/science/article/pii/S2405580821002557
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