Purification and characterization of recombinant FAD synthetase from Neurospora crassa
FAD Synthetase (FADS) [EC 2.7.7.2], the second enzyme in flavin cofactor biosynthetic pathway converts FMN to FAD, plays an important role in many redox reactions. Neurospora crassa FADS (NcFADS) was cloned and overexpressed in E. coli cells. Recombinant NcFADS was purified in high yields of ∼8 mg p...
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| Hauptverfasser: | , , , |
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| Format: | Artigo |
| Sprache: | Inglês |
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Elsevier
2021-12-01
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| Schriftenreihe: | Biochemistry and Biophysics Reports |
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| Online-Zugang: | http://www.sciencedirect.com/science/article/pii/S2405580821002557 |
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