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Efficient multi-allelic genome editing via CRISPR–Cas9 ribonucleoprotein-based delivery to Brassica napus mesophyll protoplasts

Canola (Brassica napus L.) is a valuable oilseed crop worldwide. However, trait improvement by breeding has been limited by its low genetic diversity and polyploid genetics. Whilst offering many potential benefits, the application of transgenic technology is challenged by the stringent and expensive...

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Main Authors: Sareena Sahab, Fatima Runa, Mahilini Ponnampalam, Pippa T. Kay, Elizabeth Jaya, Katerina Viduka, Stephen Panter, Josquin Tibbits, Matthew J. Hayden
Format: Artigo
Language:Inglês
Published: Frontiers Media S.A. 2024-11-01
Series:Frontiers in Plant Science
Subjects:
canola
genome editing
CRISPR
Cas9
ribonucleoprotein
protoplasts
Online Access:https://www.frontiersin.org/articles/10.3389/fpls.2024.1397632/full
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