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Efficient multi-allelic genome editing via CRISPR–Cas9 ribonucleoprotein-based delivery to Brassica napus mesophyll protoplasts

Canola (Brassica napus L.) is a valuable oilseed crop worldwide. However, trait improvement by breeding has been limited by its low genetic diversity and polyploid genetics. Whilst offering many potential benefits, the application of transgenic technology is challenged by the stringent and expensive...

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Principais autores: Sareena Sahab, Fatima Runa, Mahilini Ponnampalam, Pippa T. Kay, Elizabeth Jaya, Katerina Viduka, Stephen Panter, Josquin Tibbits, Matthew J. Hayden
Formato: Artigo
Idioma:Inglês
Publicado em: Frontiers Media S.A. 2024-11-01
coleção:Frontiers in Plant Science
Assuntos:
canola
genome editing
CRISPR
Cas9
ribonucleoprotein
protoplasts
Acesso em linha:https://www.frontiersin.org/articles/10.3389/fpls.2024.1397632/full
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