A scalable platform for efficient CRISPR-Cas9 chemical-genetic screens of DNA damage-inducing compounds

Abstract Current approaches to define chemical-genetic interactions (CGIs) in human cell lines are resource-intensive. We designed a scalable chemical-genetic screening platform by generating a DNA damage response (DDR)-focused custom sgRNA library targeting 1011 genes with 3033 sgRNAs. We performed...

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Bibliografski detalji

Glavni autori:	Kevin Lin, Ya-Chu Chang, Maximilian Billmann, Henry N. Ward, Khoi Le, Arshia Z. Hassan, Urvi Bhojoo, Katherine Chan, Michael Costanzo, Jason Moffat, Charles Boone, Anja-Katrin Bielinsky, Chad L. Myers
Format:	Artigo
Jezik:	Inglês
Izdano:	Nature Portfolio 2024-01-01
Serija:	Scientific Reports
Online pristup:	https://doi.org/10.1038/s41598-024-51735-y
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