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Impact of uniform illumination in widefield microscopy and mesoscopy

Abstract Illumination uniformity is critical for widefield optical microscopy, especially for high-throughput and accurate quantitative imaging of biological specimens. While traditional Köhler illumination improves uniformity, it often fails to deliver homogeneous intensity across large fields of v...

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Bibliografische Detailangaben
Hauptverfasser: Mete Aslan, Alexander J. Gray, Logan Packard, Caeden Giorgetta, Irving J. Bigio, M. Selim Ünlü
Format: Artigo
Sprache:Inglês
Veröffentlicht: Nature Portfolio 2025-07-01
Schriftenreihe:Scientific Reports
Schlagworte:
Online-Zugang:https://doi.org/10.1038/s41598-025-08754-0
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