Impact of uniform illumination in widefield microscopy and mesoscopy

Abstract Illumination uniformity is critical for widefield optical microscopy, especially for high-throughput and accurate quantitative imaging of biological specimens. While traditional Köhler illumination improves uniformity, it often fails to deliver homogeneous intensity across large fields of v...

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Bibliografiske detaljer

Principais autores:	Mete Aslan, Alexander J. Gray, Logan Packard, Caeden Giorgetta, Irving J. Bigio, M. Selim Ünlü
Format:	Artigo
Sprog:	Inglês
Udgivet:	Nature Portfolio 2025-07-01
Serier:	Scientific Reports
Fag:	Optical microscopy Wide-field imaging Quantitative imaging Mesoscopy Illumination uniformity Whole slide scanning
Online adgang:	https://doi.org/10.1038/s41598-025-08754-0
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