Detection of Matrix Metalloproteinase Activity by Bioluminescence via Intein-Mediated Biotinylation of Luciferase

We report bioluminescence analysis of matrix metalloproteinase (MMP) activity in biological substances using a surface-bound luciferase probe. Intein-fused luciferase protein enables site-specific biotinylation of luciferase in the presence of N-terminus cysteine-biotin via intein-mediated splicing...

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Detalhes bibliográficos

Principais autores:	Duc Long Nguyen, Hanim Kim, Dasom Kim, Jin Oh Lee, Myung Chan Gye, Young-Pil Kim
Formato:	Artigo
Idioma:	Inglês
Publicado em:	MDPI AG 2018-03-01
coleção:	Sensors
Assuntos:	luciferase bioluminescence matrix metalloproteinase intein biotinylation
Acesso em linha:	http://www.mdpi.com/1424-8220/18/3/875
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