CRISPR/Cas9 nuclease cleavage combined with Gibson assembly for seamless cloning

Restriction enzymes have two major limitations for cloning: they cannot cleave at any desired location in a DNA sequence and may not cleave uniquely within a DNA sequence. In contrast, the clustered regularly interspaced short palindromic repeat (CRISPR)–associated enzyme 9 (Cas9), when coupled with...

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Hlavní autoři:	Jia-Wang Wang, Amy Wang, Kunyu Li, Bangmei Wang, Shunqian Jin, Michelle Reiser, Richard F Lockey
Médium:	Artigo
Jazyk:	Inglês
Vydáno:	Taylor & Francis Group 2015-04-01
Edice:	BioTechniques
Témata:	CRISPR cleavage Gibson cloning large vector Cas9 single guide RNA
On-line přístup:	https://www.future-science.com/doi/10.2144/000114261
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