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CRISPR/Cas9 nuclease cleavage combined with Gibson assembly for seamless cloning

Restriction enzymes have two major limitations for cloning: they cannot cleave at any desired location in a DNA sequence and may not cleave uniquely within a DNA sequence. In contrast, the clustered regularly interspaced short palindromic repeat (CRISPR)–associated enzyme 9 (Cas9), when coupled with...

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Principais autores: Jia-Wang Wang, Amy Wang, Kunyu Li, Bangmei Wang, Shunqian Jin, Michelle Reiser, Richard F Lockey
Formato: Artigo
Idioma:Inglês
Publicado em: Taylor & Francis Group 2015-04-01
coleção:BioTechniques
Assuntos:
CRISPR cleavage
Gibson cloning
large vector
Cas9
single guide RNA
Acesso em linha:https://www.future-science.com/doi/10.2144/000114261
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