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Quantitative Fluorescence Measurement of Cardiac Na+/Ca2+ Exchanger Inhibition by Kinetic Analysis in Stably Transfected HEK293 Cells

Abstract.: We developed a method to quantitatively evaluate the potency of Na+/Ca2+ exchanger (NCX) inhibitors with fluorescence microscopy in NCX1-transfected HEK 293 cells. The reverse mode and forward mode NCX activities were measured as the ascending slope of the early phase increase in cytoplas...

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Principais autores: Namekata Iyuki, Kawanishi Toru, Iida-Tanaka Naoko, Tanaka Hikaru, Shigenobu Koki
Formato: Artigo
Idioma:Inglês
Publicado em: Elsevier 2006-01-01
coleção:Journal of Pharmacological Sciences
Acesso em linha:http://www.sciencedirect.com/science/article/pii/S1347861319344421
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