CRISPR/Cas9-Mediated Hitchhike Expression of Functional shRNAs at the Porcine <i>miR-17-92</i> Cluster

Successful RNAi applications depend on strategies allowing stable and persistent expression of minimal gene silencing triggers without perturbing endogenous gene expression. In this study, we proposed an endogenous microRNA (miRNA) cluster as a novel integration site for small hairpin RNAs (shRNAs)....

Ausführliche Beschreibung

Bibliografische Detailangaben

Hauptverfasser:	Chao Lu, Daxin Pang, Mengjing Li, Hongming Yuan, Tingting Yu, Peixuan Huang, Jianing Li, Xue Chen, Huping Jiao, Zicong Xie, Hongsheng Ouyang
Format:	Artigo
Sprache:	Inglês
Veröffentlicht:	MDPI AG 2019-02-01
Schriftenreihe:	Cells
Schlagworte:	RNAi shRNA miRNA-17-92 cluster CRISPR/Cas9 classical swine fever virus
Online-Zugang:	https://www.mdpi.com/2073-4409/8/2/113
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