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Variants of a Thermus aquaticus DNA polymerase with increased selectivity for applications in allele- and methylation-specific amplification.

The selectivity of DNA polymerases is crucial for many applications. For example, high discrimination between the extension of matched versus mismatched primer termini is desired for the detection of a single nucleotide variation at a particular locus within the genome. Here we describe the generati...

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Номзүйн дэлгэрэнгүй
Үндсэн зохиолчид: Matthias Drum, Ramon Kranaster, Christina Ewald, Rainer Blasczyk, Andreas Marx
Формат: Artigo
Хэл сонгох:Inglês
Хэвлэсэн: Public Library of Science (PLoS) 2014-01-01
Цуврал:PLoS ONE
Онлайн хандалт:http://europepmc.org/articles/PMC4011760?pdf=render
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