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Minimal genome-wide human CRISPR-Cas9 library

Abstract CRISPR guide RNA libraries have been iteratively improved to provide increasingly efficient reagents, although their large size is a barrier for many applications. We design an optimised minimal genome-wide human CRISPR-Cas9 library (MinLibCas9) by mining existing large-scale gene loss-of-f...

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Principais autores: Emanuel Gonçalves, Mark Thomas, Fiona M. Behan, Gabriele Picco, Clare Pacini, Felicity Allen, Alessandro Vinceti, Mamta Sharma, David A. Jackson, Stacey Price, Charlotte M. Beaver, Oliver Dovey, David Parry-Smith, Francesco Iorio, Leopold Parts, Kosuke Yusa, Mathew J. Garnett
Format: Artigo
Sprog:Inglês
Udgivet: BMC 2021-01-01
Serier:Genome Biology
Fag:
CRISPR-Cas9
Genome-wide
Minimal library
Organoid
KS score
Online adgang:https://doi.org/10.1186/s13059-021-02268-4
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