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Demonstration of highly efficient dual gRNA CRISPR/Cas9 editing of the homeologous GmFAD2–1A and GmFAD2–1B genes to yield a high oleic, low linoleic and α-linolenic acid phenotype in soybean

Abstract Background CRISPR/Cas9 gene editing is now revolutionizing the ability to effectively modify plant genomes in the absence of efficient homologous recombination mechanisms that exist in other organisms. However, soybean is allotetraploid and is commonly viewed as difficult and inefficient to...

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Principais autores: Phat T. Do, Cuong X. Nguyen, Hien T. Bui, Ly T. N. Tran, Gary Stacey, Jason D. Gillman, Zhanyuan J. Zhang, Minviluz G. Stacey
Formato: Artigo
Idioma:Inglês
Publicado: BMC 2019-07-01
Series:BMC Plant Biology
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Acceso en liña:http://link.springer.com/article/10.1186/s12870-019-1906-8
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