Demonstration of highly efficient dual gRNA CRISPR/Cas9 editing of the homeologous GmFAD2–1A and GmFAD2–1B genes to yield a high oleic, low linoleic and α-linolenic acid phenotype in soybean

Abstract Background CRISPR/Cas9 gene editing is now revolutionizing the ability to effectively modify plant genomes in the absence of efficient homologous recombination mechanisms that exist in other organisms. However, soybean is allotetraploid and is commonly viewed as difficult and inefficient to...

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Hauptverfasser:	Phat T. Do, Cuong X. Nguyen, Hien T. Bui, Ly T. N. Tran, Gary Stacey, Jason D. Gillman, Zhanyuan J. Zhang, Minviluz G. Stacey
Format:	Artigo
Sprache:	Inglês
Veröffentlicht:	BMC 2019-07-01
Schriftenreihe:	BMC Plant Biology
Schlagworte:	GmFAD2 Oleic acid Linoleic acid CRSIPR/Cas9 Soybean
Online-Zugang:	http://link.springer.com/article/10.1186/s12870-019-1906-8
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