Improving Properties of a Novel β-Galactosidase from Lactobacillus plantarum by Covalent Immobilization

A novel β-galactosidase from Lactobacillus plantarum (LPG) was over-expressed in E. coli and purified via a single chromatographic step by using lowly activated IMAC (immobilized metal for affinity chromatography) supports. The pure enzyme exhibited a high hydrolytic activity of 491 IU/mL towards o...

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Detalhes bibliográficos

Principais autores:	Rocio Benavente, Benevides C. Pessela, Jose Antonio Curiel, Blanca de las Rivas, Rosario Muñoz, Jose Manuel Guisán, Jose M. Mancheño, Alejandra Cardelle-Cobas, Ana I. Ruiz-Matute, Nieves Corzo
Formato:	Artigo
Idioma:	Inglês
Publicado em:	MDPI AG 2015-04-01
coleção:	Molecules
Assuntos:	β-galactosidase Lactobacillus plantarum immobilization glyoxyl-agarose oligosaccharides synthesis lactose lactulose
Acesso em linha:	http://www.mdpi.com/1420-3049/20/5/7874
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