Targeted large-fragment genomic deletion in human pluripotent stem cells (hPSCs) via CRISPR/Cas9

Large genomic deletions are increasingly recognized as contributors to human disease. Here, we describe a CRISPR/Cas9-based protocol to induce targeted large-fragment deletions in human pluripotent stem cells (hPSCs). Two sgRNAs flanking the target region were designed, synthesized, and co-delivered...

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Dades bibliogràfiques

Autors principals:	Sai Wei, Ruiting Ma, Ting Zhang, Luan Wen
Format:	Artigo
Idioma:	Inglês
Publicat:	Elsevier 2025-12-01
Col·lecció:	Stem Cell Research
Matèries:	CRISPR/Cas9 RNP Large-fragment deletion editing
Accés en línia:	http://www.sciencedirect.com/science/article/pii/S1873506125002090
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