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Protocol for live imaging of bacteria-cell interactions in genetically modified mouse small intestinal organoids

Summary: Here, we present a protocol for microinjection of bacteria into mouse small intestinal organoids that recapitulates the natural route of infection of intestinal epithelial cells from the intestinal lumen. We describe steps for visualizing bacteria-cell interactions by live imaging of infect...

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Bibliographic Details
Main Authors: Minhee Kim, Cindy Fèvre, Morgane Lavina, Olivier Disson, Marc Lecuit
Format: Artigo
Language:Inglês
Published: Elsevier 2024-03-01
Series:STAR Protocols
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Online Access:http://www.sciencedirect.com/science/article/pii/S2666166723007402
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