Protocol to generate genetically engineered organoid-initiated mouse models of esophageal cancer

Summary: Here, we present a protocol for generating genetically engineered organoid-initiated mouse models of esophageal cancer. We describe steps for isolating mouse esophageal primary cells, organoids culture, construct and virus preparation, and genome editing. We then detail procedures for trans...

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Detalhes bibliográficos

Principais autores:	Jian Wang, Jiajia Du, Xiangmeng Luo, Linjie Guo, Xiang Li, Ziling Dong, Zhenghao Lu, Xudong Wan, Lu Wang, Hongyu Liu, He Li, Ping Tan, Yanhong Ji, Yuan Li, Yaxin Li, Yushang Yang, Yong Yuan, Longqi Chen, Bing Hu, Yu Liu, Feifei Na, Chong Chen
Formato:	Artigo
Idioma:	Inglês
Publicado em:	Elsevier 2025-09-01
coleção:	STAR Protocols
Assuntos:	Cancer Model Organisms Organoids
Acesso em linha:	http://www.sciencedirect.com/science/article/pii/S2666166725004344
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