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An improved CRISPR-Cas9 protein-based method for knocking out insect Sf9 cell genes

Abstract Insect cells are one of the uprising expression systems in the biopharmaceutical industry to produce vaccines and gene therapy vectors, but cell line development has been limited by the lack of established genetic engineering tools and genomic characterization. CRISPR-Cas9 has arisen as a p...

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Principais autores: Miguel Graça, Nikolaus Virgolini, Ricardo Correia, Jose Escandell, António Roldão
Format: Artigo
Sprog:Inglês
Udgivet: Springer 2026-01-01
Serier:Applied Microbiology and Biotechnology
Fag:
Spodoptera frugiperda
Baculovirus expression vector system
Cell line development
CRISPR-Cas9
Apoptosis
Advanced therapy medicinal products
Online adgang:https://doi.org/10.1007/s00253-026-13722-3
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