A protocol for the generation of patient-specific iPSC lines from peripheral blood mononuclear cells

Summary: The current procedure thoroughly explains how to reprogram induced pluripotent stem cells (iPSCs) from the patient’s peripheral blood mononuclear cells (PBMCs), a less invasive source; e.g., somatic cells. We describe how to isolate PBMCs and reprogram them into iPSCs by electroporation. Fu...

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Detalhes bibliográficos

Principais autores:	Langping Gao, Fangqin Wang, Yan Wang, Lidan Hu, Jianhua Mao
Formato:	Artigo
Idioma:	Inglês
Publicado em:	Elsevier 2022-09-01
coleção:	STAR Protocols
Assuntos:	Cell Biology Health Sciences Stem Cells
Acesso em linha:	http://www.sciencedirect.com/science/article/pii/S2666166722004105
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