A protocol for the generation of patient-specific iPSC lines from peripheral blood mononuclear cells

Summary: The current procedure thoroughly explains how to reprogram induced pluripotent stem cells (iPSCs) from the patient’s peripheral blood mononuclear cells (PBMCs), a less invasive source; e.g., somatic cells. We describe how to isolate PBMCs and reprogram them into iPSCs by electroporation. Fu...

ver descrição completa

Detalhes bibliográficos

Principais autores:	Langping Gao, Fangqin Wang, Yan Wang, Lidan Hu, Jianhua Mao
Formato:	Artigo
Idioma:	Inglês
Publicado em:	Elsevier 2022-09-01
Colecção:	STAR Protocols
Assuntos:	Cell Biology Health Sciences Stem Cells
Acesso em linha:	http://www.sciencedirect.com/science/article/pii/S2666166722004105
Tags:	Adicionar Tag Sem tags, seja o primeiro a adicionar uma tag!