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Protocol for the derivation and culture of murine trophoblast organoids for CRISPR-Cas9 screening

Summary: Murine trophoblast organoids present a more balanced array of trophoblast subtypes, rendering them a suitable platform for CRISPR-Cas9-based screening. Here, we present a protocol for the derivation and culture of murine trophoblast organoids from trophoblast stem cells or placentae. We des...

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Bibliografische Detailangaben
Hauptverfasser: Qian Mao, Jingwei Jiang, Qinying Ye, Haopeng Wang, Chao-Po Lin
Format: Artigo
Sprache:Inglês
Veröffentlicht: Elsevier 2024-12-01
Schriftenreihe:STAR Protocols
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Online-Zugang:http://www.sciencedirect.com/science/article/pii/S2666166724005707
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