Protocol for the derivation and culture of murine trophoblast organoids for CRISPR-Cas9 screening

Summary: Murine trophoblast organoids present a more balanced array of trophoblast subtypes, rendering them a suitable platform for CRISPR-Cas9-based screening. Here, we present a protocol for the derivation and culture of murine trophoblast organoids from trophoblast stem cells or placentae. We des...

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Bibliografski detalji

Glavni autori:	Qian Mao, Jingwei Jiang, Qinying Ye, Haopeng Wang, Chao-Po Lin
Format:	Artigo
Jezik:	Inglês
Izdano:	Elsevier 2024-12-01
Serija:	STAR Protocols
Teme:	High-Throughput Screening CRISPR Stem Cells Cell Differentiation Organoids
Online pristup:	http://www.sciencedirect.com/science/article/pii/S2666166724005707
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