Methyl β-Cyclodextrin-sperm-mediated gene editing (MBCD-SMGE): a simple and efficient method for targeted mutant mouse production

Abstract Background Generating targeted mutant mice is a crucial technology in biomedical research. This study focuses on optimizing the CRISPR/Cas9 system uptake into sperm cells using the methyl β-cyclodextrin-sperm-mediated gene transfer (MBCD-SMGT) technique to generate targeted mutant blastocys...

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Autori principali:	Parisa Moradbeigi, Sara Hosseini, Mohammad Salehi, Asghar Mogheiseh
Natura:	Artigo
Lingua:	Inglês
Pubblicazione:	BMC 2024-01-01
Serie:	Biological Procedures Online
Soggetti:	Sperm-mediated gene Transfer (SMGT) CRISPR/Cas9 Methyl β-cyclodextrin (MBCD) Targeted mutant mouse Reactive oxygen species (ROS) Sperm-mediated gene editing (SMGE)
Accesso online:	https://doi.org/10.1186/s12575-024-00230-9
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