Methyl β-Cyclodextrin-sperm-mediated gene editing (MBCD-SMGE): a simple and efficient method for targeted mutant mouse production

Abstract Background Generating targeted mutant mice is a crucial technology in biomedical research. This study focuses on optimizing the CRISPR/Cas9 system uptake into sperm cells using the methyl β-cyclodextrin-sperm-mediated gene transfer (MBCD-SMGT) technique to generate targeted mutant blastocys...

Disgrifiad llawn

Manylion Llyfryddiaeth

Prif Awduron:	Parisa Moradbeigi, Sara Hosseini, Mohammad Salehi, Asghar Mogheiseh
Fformat:	Artigo
Iaith:	Inglês
Cyhoeddwyd:	BMC 2024-01-01
Cyfres:	Biological Procedures Online
Pynciau:	Sperm-mediated gene Transfer (SMGT) CRISPR/Cas9 Methyl β-cyclodextrin (MBCD) Targeted mutant mouse Reactive oxygen species (ROS) Sperm-mediated gene editing (SMGE)
Mynediad Ar-lein:	https://doi.org/10.1186/s12575-024-00230-9
Tagiau:	Ychwanegu Tag Dim Tagiau, Byddwch y cyntaf i dagio'r cofnod hwn!