Exploitation of a Type 1 Toxin–Antitoxin System as an Inducible Counter-Selective Marker for Genome Editing in the Acetogen <em>Eubacterium limosum</em>

Targeted mutations in the anaerobic methylotroph <i>Eubacterium limosum</i> have previously been obtained using CRISPR-based mutagenesis methods. In this study, a RelB-family toxin from <i>Eubacterium callanderi</i> was placed under the control of an anhydrotetracycline-sensitive promoter, forming a...

Täydet tiedot

Bibliografiset tiedot

Päätekijät:	James Millard, Alexander Agius, Ying Zhang, Philippe Soucaille, Nigel Peter Minton
Aineistotyyppi:	Artigo
Kieli:	Inglês
Julkaistu:	MDPI AG 2023-05-01
Sarja:	Microorganisms
Aiheet:	<i>Eubacterium limosum</i> <i>Eubacterium callanderi</i> toxin–antitoxin systems RelBE CRISPR gene editing
Linkit:	https://www.mdpi.com/2076-2607/11/5/1256
Tagit:	Lisää tagi Ei tageja, Lisää ensimmäinen tagi!