Exploitation of a Type 1 Toxin–Antitoxin System as an Inducible Counter-Selective Marker for Genome Editing in the Acetogen <em>Eubacterium limosum</em>
Targeted mutations in the anaerobic methylotroph <i>Eubacterium limosum</i> have previously been obtained using CRISPR-based mutagenesis methods. In this study, a RelB-family toxin from <i>Eubacterium callanderi</i> was placed under the control of an anhydrotetracycline-sensitive promoter, forming a...
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| Principais autores: | , , , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
MDPI AG
2023-05-01
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| coleção: | Microorganisms |
| Assuntos: | |
| Acesso em linha: | https://www.mdpi.com/2076-2607/11/5/1256 |
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